Table 1.
List of primers
| Gene | Direction | Primer sequence 5′→3′ |
|---|---|---|
| Tat –C |
Forward |
GGAATTCCATATGATGGAGCCAGTAGATCC |
| Reverse |
CCGCTCGAGATCGAATGGATCTGTCTTTG. |
|
| IFNB1 |
Forward |
GCTCTCCTGTTGTGCTTCTCCAC |
| Reverse |
CAATAGTCTCATTCCAGCCAGTGC |
|
| IRF7 |
Forward |
GCTGGACGTGACCATCATGTA |
| Reverse |
GGGCCGTATAGGAACGTGC |
|
| Beta-actin |
Forward |
TCATGAAGTGTGACGTGGAC |
| Reverse |
CAGGAGGAGCAATGATCTTGAT |
|
| TRAF3 |
Forward |
GCGTGTCAAGAGAGCATCGTT |
| Reverse |
GCAGATGTCCCAGCATTAACT-3 |
|
| IRF3 |
Forward |
GTGGCCTGGGTGAACAAGAG |
| Reverse |
TGGAAGATTCCGAAATCCTCCT |
|
| MUT TRAF3 3′ UTR |
Forward |
CAACAAGATAAATGCTGTCAGAGAAGG3′ |
| Reverse | CCTTCTCTGACAGCATTTATCTTGTTG 3′ |