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Effect of anti-HGF neutralizing antibody on PC-3 cell functions PC-3 cells were treated with 10 μg/ml of the neutralizing antibody (anti-HGF) or control antibody (nIgG) for 4 days (A), 14 days (B) or 1 day (C), for the detection of cell growth (MTT assay, A), clonogenicity (colony formation assay, B), or cell migration [“wound-healing” assay, C (magnification, ×5)]. Columns, mean; bars, SD (n = 6). Data represent ≥3 independent experiments.
