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. Author manuscript; available in PMC: 2012 Aug 14.
Published in final edited form as: Br J Haematol. 2010 Mar 12;149(4):537–549. doi: 10.1111/j.1365-2141.2010.08127.x

Fig. 2. AS703026 specifically blocked ERK1/2 activation in MM cells, cultured alone or with BMSCs.

Fig. 2

A, U266 cells were incubated with DMSO or AS703026 (5-2000 nM) for 1h (left) or 200 nM for 0-72h (right) and then subjected to immunoblotting. B, MM cell lines cultured alone or with BMSCs were treated with (+) or without (-) AS703026 (5 μM for MM1S; 0.5 μM for H929) overnight, followed by immunoblotting of cell lysates with indicated Abs. Anti-α-tubulin was used as a loading control.