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A detail of 2DE maps for AS-3-stored RBC membranes at day 0, 21 and 42 (from left to right). The grey ellipse, black rectangle and white rectangle with rounded edges indicate a high MW region, average MW region with medium to high pI and a low MW region, respectively. As storage progresses, fragmentation of proteins from the grey ellipsoid region increases the number of spots at low MW. A right-shift (increase in apparent pI) can be observed for most of the spots by storage day 42.
