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. Author manuscript; available in PMC: 2012 Aug 14.
Published in final edited form as: Free Radic Biol Med. 2010 Jul 16;49(8):1255–1262. doi: 10.1016/j.freeradbiomed.2010.07.006

Figure 3. Proliferation of Sod2−/− cells and mitochondrial function.

Figure 3

(A) Wild-type (solid line with open circle (○)) and Sod2−/− cells (dotted line with solid circle (●)) were seeded equally onto 96-well plates as described in the Materials and Methods section. Cell growth was monitored by MTT assay every 24 hours. Data represent three different experiments. *p < 0.05 between wild-type and Sod2−/− MEFs at each time point. (B) ATP production measured in whole cell. **p < 0.01 between wild-type and Sod2−/− MEFs. (C) Oxygen consumption (OCR) measured by Seahorse Bioscience Extracellular Flux Analyzer XF24. *p < 0.05 between wild-type and Sod2−/− MEFs treated with FCCP; **p < 0.05 between untreated and FCCP-treated cells. WT: wild type; KO: Sod2−/− MEF.