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. Author manuscript; available in PMC: 2012 Aug 14.
Published in final edited form as: Bone. 2010 Jan 22;46(5):1380–1390. doi: 10.1016/j.bone.2010.01.373

Figure 4. Spatial and Temporal changes in gene expression (mean ± SEM) of transcription factors implicated in growth plate cartilage.

Figure 4

(A) Relative expression of mRNA was measured by expression microarray (Affymetrix Rat Genome Array 230 2.0). Spatially, mRNA in different zones of growth plate of 1 wk old rats was compared. Temporally, mRNA in proliferative zone at 3, 6, 9 and 12 wk old rats was compared. The data was background corrected and normalized using the MAS5 statistical algorithm. *, P < 0.05 compared with the preceding zone; NS, not significant (B) For significant spatial changes, the relative expression of c-Fos, Hif1a, and Atf3 was verified using quantitative real-time PCR. RZ, resting zone; PZ, proliferative zone; HZ, hypertrophic zone.