Figure 4.

SER-5 is essential for 5-HT-dependent increases in sensitivity to dilute octanol. A, Effect of ser-5 RNAi on sensitivity to dilute (30%) octanol in both wild-type and rrf-3(pk1269) animals. B, Wild-type and ser-5 null animals were examined for aversive responses to dilute octanol in the presence or absence of 5-HT (4 mm), as described in Materials and Methods. In addition, ser-5 expression in the ASH sensory neurons was knocked down in wild-type animals (sra-6p::ser-5(RNAi) animals). Data are presented as mean ± SE and analyzed by two-tailed Student's t test. ***p < 0.001, significantly different from wild-type or rrf-3(pk1269) animals under identical test conditions. The number of trials is indicated under the bars and results from rescued ser-5 null animals are indicated by hatched bars.