Table 2.

Neuroprotective effects of 4-phenylcoumarins (200 μM) isolated from hexane bark of M. kunstleri against H₂O₂-induced apoptosis in NG108-15 cells.

Compounda	Cell viability (%)b
Control	100
H2O2 c	45.41 ± 3.42#
1	42.41 ± 2.35*
2	51.46 ± 1.42*
3	78.99 ± 3.49*
4	61.99 ± 1.58*
5	56.47 ± 1.70*
EGCG (50 μM)d	85.19 ± 2.17

The values shown are the means ± SE of at least three independent experiments.

^aNG108-15 cells were pretreated with 4-phenylcoumarins (3.125–200 μM) for 2 h prior to exposure to H₂O₂ (2 mM). After incubation, cells were assessed by MTT to determine the percentage viability.

^bCell viability was measured by MTT assay.

^cH₂O₂-treated value differed significantly from the untreated control at the level of ^# P < 0.05.

^dEGCG was used as standard positive control.

^#Results differ significantly from H₂O₂-treated group compared to the control untreated group: P < 0.05.

^∗Significantly from H₂O₂-treated group compared to the treatment group: P < 0.05.