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. 2012 Jul 22;2012:785743. doi: 10.1155/2012/785743

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Copyright © 2012 Suzana Makpol et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Contour diagram of FITC-Annexin V/PI double staining by flow cytometry. The three quadrants represent different cells conditions: the upper right quadrant (R1); nonviable, late apoptotic and necrotic cells (FITC⁺/PI⁺), lower left quadrant (R2); viable cells (FITC⁻/PI⁻), and lower right quadrant (R3); early apoptotic cells (FITC⁺/PI⁻) (a). Percentage of cells at early apoptotic stage demonstrated by FITC⁺/PI⁻ (b). Data are expressed as means ± SD, n = 6. ^adenotes P < 0.05 compared to control young, ^b P<0.05 compared to GTT, and ^c P<0.05 compared to SIPS.