Fig 1.

Increased mortality of MyD88-deficient mice during C. difficile colitis. (A) MyD88^−/− and C57BL/6 (B6) mice without prior antibiotic treatment were challenged with a high dose of C. difficile VPI10463 spores (10⁵) and followed for weight loss. Results are representative of two independent experiments (both with n = 4 or 5/group). (B to D) Mice received clindamycin on day −1 and were infected with C. difficile VPI10463 spores on day 0. Mice (n = 10/group) were followed for weight loss (B) and survival (C). The log-rank (Mantel-Cox) test was used for statistical analysis of survival. Results are representative of 2 independent experiments. (D) C. difficile burden in the cecum was assessed 2 days postchallenge in MyD88^−/− and wild-type mice. Results are pooled from two independent experiments (B to E). (E) Mice received a single dose of clindamycin and were challenged with 10⁴ spores of C. difficile strain CD196.