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BTg01Z.A response to the fractionated single clones is antigen specific and MHC restricted. (A) A positive pool, pTg778, from the initial screen was further fractionated until a single stimulatory clone was identified and sequenced. The BTg01Z.A response to individual bacterial colonies isolated from pool 778 was tested (10 representative clones are shown). Clone 778.76 was selected for further analysis. (B and C) BTg01Z.A response was antigen specific and MHC restricted.
