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Intracellular survival of wild-type K96243 and isogenic mutants. J774A.1 murine macrophages were infected with wild-type K96243 and isogenic mutants at an MOI of 10, and intracellular bacterial numbers were determined at the indicated time points after initial infection by plating onto LB agar plates. Bars and error bars represent the means and standard deviations of a representative experiment performed in three technical replicates for each time point.
