Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| B. pseudomallei strains | ||
| K96243 | Clinical isolate from Thailand | 23 |
| K96243 ΔrelA | Inactivation of BPSL1946 (relA) by complete deletion | This study |
| K96243 ΔrelA ΔspoT | Inactivation of BPSL1946 (relA) and BPSL2561 (spoT) by complete deletion | This study |
| 576 | Clinical isolate from Thailand | 2 |
| 2D2 | Auxotroph transposon mutant of B. pseudomallei 576 | 3 |
| E. coli strains | ||
| DH5 Δpir | recA1 gyrA (Nal) Δ(lacIZYA-argF) (ϕ80dlacΔ[lacZ]M15) pirRK6 | 43 |
| S17-1 Δpir | RPA-2 tra regulon; pirRK6 Smr Tpr | 43 |
| Plasmids | ||
| pDM4 | Suicide vector with R6K origin; Cmr | 29 |
| pDM4-ΔrelA | 600 bp up- and downstream of relA cloned into pDM4 via XhoI/SphI sites | This study |
| pDM4-ΔspoT | 600 bp up- and downstream of spoT cloned into pDM4 via SalI/XmaI sites | This study |