Table 2.
Oligonucleotides used in this study
| Name | Sequence (5′–3′)a | Purpose |
|---|---|---|
| relA-1-fw | CCCCTCGAGGAAGGCGCGCGGACAAGCGG | Mutagenesis |
| relA-2-rv | CGGCGCTCACTGCAGCATCGTCGGGTATGGCCAGTT | Mutagenesis |
| relA-3-fw | CCGACGATGCTGCAGTGAGCGCCGCCGGTGGGCCGC | Mutagenesis |
| relA-4-rv | CCCGCATGCCGTATCGACGAGTTCGCCGTC | Mutagenesis |
| spoT-1-fw | GCGGTCGACCTGCCGCCGTCGCTCGCGGC | Mutagenesis |
| spoT-2-rv | GCGGCGCTAACTAGTCATTTTCGCCTCCTGGGTTCG | Mutagenesis |
| spoT-3-fw | GCGAAAATGACTAGTTAGCGCCGCGCGGCGCCCGAC | Mutagenesis |
| spoT-4-rv | GCGCCCGGGGCCTGCGCGAAATCGAGGTCG | Mutagenesis |
| relA-up-fw | ACAACGGCACCGTCTATCTC | Confirmation of plasmids |
| relA-down-rv | GCGGTGTCATAACCGTATTC | Confirmation of plasmids |
| spoT-up-fw | CTCGCATTACCGTTGAAGAC | Confirmation of plasmids |
| spoT-down-rv | GCCCTTTCAGTTCGAAGTTG | Confirmation of plasmids |
| relA-3-fw | GCGTGCAGTCGGACCTGCTG | Confirmation of mutation |
| relA-4-rv | GTTCGGGAAAATTCGCAAGCC | Confirmation of mutation |
| relA-5-wt | GTGGAAGCGGAATCGGCTG | Confirmation of mutation |
| spoT-3-fw | GGTGAAGAAGCAGTTCCGCA | Confirmation of mutation |
| spoT-4-rv | CGCCCGAAACAAATGGCGG | Confirmation of mutation |
| spoT-5-wt | GCGGACGATGGTGTAGTGC | Confirmation of mutation |
| relA-RT-1 | AGATCCGCACGCAGGAAATG | RT-PCR analysis |
| relA-RT-2 | TTCGCTGTGCAGGTGGTAAG | RT-PCR analysis |
| spoT-RT-1 | GCGCATCAATGGCTCAAGTC | RT-PCR analysis |
| spoT-RT-2 | TTCAGGCGCATCGTCTTCAG | RT-PCR analysis |
| 16S-RT-1 | GCCAGTCACCAATGCAGTTC | RT-PCR normalization |
| 16S-RT-2 | ACCAAGGCGACGATCAGTAG | RT-PCR normalization |
a
Restriction sites are underlined. Complementary regions are in italics. Translational start and stop codons are in bold.