Fig 3.
Generation of the Δbbb22-23 mutant in B. burgdorferi. (A) Schematic representation of the wild type (WT) and Δbbb22-23 loci on cp26. The sequence of the BBB22-23 open reading frame was replaced with a flaBp-aadA antibiotic resistance cassette. Locations of primers for analysis of the mutant clones are indicated with small arrows and labels P1 to P6. Primer sequences are listed in Table 1. The boundaries of the deletion construct are indicated by broken lines. (B) PCR analysis of mutant clones. Genomic DNA isolated from WT and Δbbb22-23 spirochetes served as the template DNA for PCR analyses. DNA templates are indicated across the top of the gel image. The oligonucleotide pairs used to amplify specific DNA sequences are indicated below the gel image and correspond to target sequences as shown in panel A.