Table 1.
Primers and probes used in this study
| Primer or probe | Designation | Sequence (5′–3′) |
|---|---|---|
| 1 | BBB22 − 500 | CAGTTAAAGAGCTTACAAGCCC |
| 2 | BBB23 + 500 | AGGATACCTTGCAATATGATCC |
| 3 | aadA-3′ Xho1 | CCGCTCGAGTTATTTGCCGACCTACCTTGG |
| 4 | flaB- 5′ Xho1 | CCGCTCGAGCTGTCGCCTCTTGTGGCTTC |
| 5 | BBB22-3′ HindIII | CCCAAGCTTGACTCTTTTTTATGATTTATAATTTAGG |
| 6 | BBB23-5′ BamHI | CGGGATCCTATTTTCAAACTTTACCTGACAGCG |
| 7 | BBB22F (17918) Sall 3′ | ACGCGTCGACCCTAAATTATAAATCATAAAAAAGAGTC |
| 8 | BBB23R (20824) Sall 5′ | ACGCGTCGACAATAATTATTAGGCTTTTAATTCTTTTTAAAGGCC |
| 9 | BBB22-Fwd TaqMan | AAGAAATTATCGGTGGTATTACCACTTT |
| 10 | BBB22-Rev TaqMan | CAATTGGCATACCTGTGCTAGATAATAT |
| 11 | BBB23-Fwd TaqMan | GCACTAGTTACCGCAACCTGTCT |
| 12 | BBB23-Rev TaqMan | CTCATTCCAGAAGCCAATGCT |
| 13 | BBB22 Probe | AGCATGGCATACATAATAGCTGTTAATCCGGC |
| 14 | BBB23 Probe | CTAATGGGACTTTATACCAATACGCCTT |