Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or phenotype | Background or resistance | Reference |
|---|---|---|---|
| Strains | |||
| M1511 | TTSS-1− (invC::aphT); Smr Kmr | M1525 | 43 |
| M1525 | S. Enteritidis 125109 wild type; Smr | Wild type | 42 |
| SB566 | TTSS-1− (invC::aphT); Smr Kmr | SB300 | 38 |
| Z290 | SPI-1-deficient SEN1140 mutant (invC::aphT SEN1140::cat); Smr Kmr Cmr | M1511 | This study |
| Z291 | SEN1140 mutant (SEN1140::cat); Smr Cmr | M1525 | This study |
| Z292 | Z290 complemented with SEN1140 through pCH112; Ampr | Z290 | This study |
| Plasmids | |||
| pM968 | Promoterless GFPmut2 in promoterless pBAD24 vector | Ampr | 41 |
| pM975 | bla PssaG GFPmut2 plasmid with oripMB1 | Ampr | 28 |
| pM973 | bla PssaH GFPmut2 plasmid with oripMB1 | Ampr | 40 |
| pCH112 | hilA ORF cloned into PBAD/myc-His; oripBR322 | Ampr | 28 |
| pKD3 | bla FRT cat FRT PS1 PS2 oriR6K | Cmpr | 25 |
| pKD46 | bla PBAD gam bet exo pSC101 oriTS | Ampr | 25 |
| pM2152 | 450 bp upstream (P0999) of SEN0999 cloned into pM968 between BamHI and XbaI | Ampr | This study |
| pM2153 | 500 bp upstream (P4250) of SEN4250 cloned into pM968 between BamHI and XbaI | Ampr | This study |
| pM2154 | 550 bp upstream (P1013) of SEN1013 cloned into pM968 between BamHI and XbaI | Ampr | This study |
| pM2155 | 750 bp upstream (P1140) to SEN1140 cloned into pM968 between BamHI and XbaI | Ampr | This study |
| pM2156 | 650 bp upstream (P1975) to SEN1975 cloned into pM968 between BamHI and XbaI | Ampr | This study |
| pM2157 | 700 bp upstream (P1994) to SEN1994 cloned into pM968 between BamHI and XbaI | Ampr | This study |
| pM2158 | SEN1140 ORF with 750-bp upstream region cloned into pCH112 between NcoI and XbaI; oripBR322 | Ampr | This study |