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Detection of lysine succinylation in core histones of different species. A, chemical structure and hypothesized mechanism for lysine succinylation. B, Western blot analysis (top panel) of core histones from S. cerevisiae, D. melanogaster, M. musculus, and H. sapiens cells with or without competition of a peptide library bearing a fixed unmodified lysine (K) or succinylated lysine (KSUCC). The bottom panel shows the loading control.
