Fig. 3.
HPLC and mass spectrometric verification of histone succinylation at H4K31 (DNIQGITK+100.0150 PAIR). A, high resolution MS/MS spectra of H4K31 succinylation peptide (DNIQGITK+100.0150 PAIR) from affinity-enriched HeLa histone extract using anti-succinyllysine pan antibody (top), the synthetic DNIQGITKsuccPAIR (middle), and the mixture of them (bottom). The insets show the precursor ions. The label Δ designates b or y ions with water and/or ammonia loss. B, extracted ion chromatograms of the in vivo derived H4K31 peptide (top), its synthetic counterpart (middle), and their mixture (bottom). C, MS and MS/MS spectrum of D4-succinyllysine peptide.