Fig. 4.
HPLC and mass spectrometric verification of histone succinylation at H4K31 (EIAQDFK+100.0152TDLR). A, high resolution MS/MS spectra of in vivo H3K79 succinylation peptide (EIAQDFK+100.0152TDLR) from affinity-enriched HeLa histone extract (top), the synthetic EIAQDFKsuccTDLR (middle), and the mixture of them (bottom). B, extracted ion chromatograms of the in vivo derived H3K79 peptide (top), its synthetic counterpart (middle), and their mixture (bottom). C, MS and MS/MS spectrum of D4-succinyllysine peptide.