Fig. 7.

Lysate concentration, but not FG nup position, number of FG repeats, or amino acid composition, correlates with measured affinity. Kap/FG nup affinities as measured in vitro show little apparent correlation with location in the NPC (A), number of FG repeats (B), or FG nup amino acid composition (C) in the presence (shades of red) or absence (shades of blue) of lysate. The palest points are Kap95, medium points are Kap121, and the darkest points are Kap123. (*) identifies points corresponding to Nup53 and Nup59, which contain few FG repeats and are not always considered to be true FG nups.