Fig. 1.

Rational construction of lymphoma super-SILAC mix. A, label-free proteomics of nine B-cell lymphoma cell lines was performed after FASP-SAX processing and analyzed using high resolution precursor and fragment measurements on an Orbitrap Velos. They included two Hodgkin lymphoma cell lines (L428 and L1236) and seven non-Hodgkin lymphoma cell lines (Ramos, Mutu, BL-41, OciLy3, U2932, BJAB, and DB). B, PCA of nine B-cell lymphoma cell lines based on their protein expression profiles. The red boxes indicate cell lines selected for the super-SILAC mix. The gray dashed ellipse groups non-Hodgkin lymphoma cell lines to be further analyzed by a second PCA. C, PCA of the six non-Hodgkin lymphoma cell lines encircled in B. The red boxes indicate cell lines selected for the super-SILAC mix.