Photothermal treatment of mouse tumor using intravenous injection of ICG and ICG-PL-PEG-mAb, followed by laser irradiation.
A. Photograph of a mouse bearing U87-MG tumor.
B. Thermographic images of mice bearing U87-MG tumors under different treatments.
C. Plot of maximum surface temperature of the irradiated area as a function of the irradiation time. (n=3 per group).
D. Histological staining of the excised tumors 12 hours after different treatments. Distinctive characteristics of cellular damage were observed in the Laser+ICG-PL-PEG-mAb treated tumors, including coagulative necrosis (arrow), abundant pyknosis (arrowhead) and considerable regions of karyolysis (asterisk).
E. Time-dependent tumor growth curves of U87-MG tumor. The results were presented as the arithmetic means with standard deviations of tumor volumes in each group. Only the Laser+ICG-PL-PEG-mAb treated group shows significant suppression of tumor growth compared with other experimental groups (n=3).