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. 2012 Jul 4;32(27):9133–9142. doi: 10.1523/JNEUROSCI.0996-12.2012

Figure 6.

Figure 6.

Accumulation of pTDP-43 and increased caspase 3 activation in differentiated BE(2)M17 cells expressing ataxin 2 with intermediate-length polyQ expansion. BE(2)-M17 neuroblastoma cells transfected with empty vector or ataxin 2 with increasing polyQ length (22Q, 31Q, or 39Q) were differentiated for 5 d and heat shocked for 1 h at 42°C, or control for 1 h at 37°C, then lysed into soluble and insoluble fractions. A, An accumulation of insoluble pTDP-43 C-terminal fragment was seen with ataxin 2 31Q compared with empty vector or ataxin 2 22Q and 39Q. There was also an increase in cleaved caspase 3 in cells expressing 31Q, but not 22Q or 39Q. B, Quantification from three independent experiments (pTDP-43 levels normalized to actin and compared with empty vector control; error bars represent SD; *p < 0.05). C, Quantification from three independent experiments (cleaved caspase 3 levels normalized to actin and compared with empty vector control; error bars represent SD; *p < 0.05).