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. 2012 Jun;9(2):130–136. doi: 10.3724/SP.J.1263.2011.11261

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Institute of Geriatric Cardiology

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Figure 4. — (A): Representative photomicrographs (× 200) of heart sections from rats subjected to sham myocardial ischemia, ischemia/reperfusion (30 minutes/2 hours) treated with vehicle, or ischemia/reperfusion (30 minutes/2 hours) treated with GXST are shown. TUNEL staining (green) indicates apoptotic nuclei; DAPI counterstaining (blue) indicates total nuclei. (B): Quantification of apoptotic nuclei (n = 5). Data are presented as mean ± SE. TUNEL-positive nuclei are expressed as a percentage of the total number of nuclei, automatically counted and calculated using Image-Pro Plus software. ^#P < 0.05 vs. Sham, *P < 0.05 vs. Control.