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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Clin Chem. 2012 May 15;58(7):1130–1138. doi: 10.1373/clinchem.2012.183095

Figure 1.

Figure 1

Temperature tolerant-full-COLD PCR (TT-full-COLD PCR) principle. Each TT-full-COLD-PCR step is repeated X times (X=7 herein), following which a new step at a critical denaturation temperature (Tc) incremented by 0.5°C is applied. In the present work, there are a total of 5 steps corresponding to a combined Tc window of 2.5°C.