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Application of a single TT-full-COLD PCR thermocycling program to enrich mutations in exons 6–9 in TP53, using known serial dilutions into wild-type DNA. Sanger sequencing chromatograms depict results from conventional PCR (A) and TT-full-COLD-PCR amplification (B). The left and right columns in frame A correspond to the left and right columns in frame B.
