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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Clin Chem. 2012 May 15;58(7):1130–1138. doi: 10.1373/clinchem.2012.183095

Figure 4.

Figure 4

High resolution melt analysis (HRM) and sequencing after conventional PCR and TT-full- COLD PCR for TP53 exon 6 (C>T mutation) and exon 8 (G>C mutation), using known serial dilutions into wild-type DNA. Following conventional PCR, the 3% exon 6 mutation is detected via HRM but not via sequencing; the 3% exon 8 mutation is detected via neither method. Following TT-full-COLD these mutations are detected with both HRM and sequencing, although in exon8 the enrichment is marginally discriminated from background.