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. 2007 Mar 1;1(2):80–91. doi: 10.4161/chan.3999

Figure 1.

Figure 1.

Mutation of the NH2‑terminal RKR motif compromises function of hIK1. Representative whole--cell current traces from (A) HA‑hIK1 or (B) RKR/AAA stably transfected in HEK293 cells in response to 10 mM DCEBIO and 3 mM clotrimazole. (C) Average DCEBIO‑stimulated (10 mM), clotrimazole‑sensitive (3 mM) current densities (pA/pF) plotted for each construct at ‑20 mV. The number of experiments is indicated in parenthesis. The asterisks indicate -statistical significance (p < 0.05). (D) Cell Surface Immunoprecipitation (CS‑IP) of HA‑hIK1 channel constructs. (Top panel) CS‑IP confirms expression of HA‑hIK1, R13A/R14A, R15A, K16A, R17A, RKR/AAA, R15A/K16A and R15A/R17A at the cell surface of HEK293 cells. (Bottom panel) Immunoblot (20 mg total protein) indicating the cellular levels of IK1 channel expression for all constructs. The immunoblots shown are representative of three separate experiments.