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. 2007 Mar 1;1(2):80–91. doi: 10.4161/chan.3999

Figure 2.

Figure 2.

Reduced incubation temperature increases expression but not -function of RKR/AAA‑HA‑hIK1. (A, left panel) Cell surface -immunoprecipitation of HA‑hIK1 (lane 1), RKR/AAA grown at 37°C (lane 2), RKR/AAA grown at 27°C (lane 3), RKR/AAA grown at 27°C in the presence of 5 mM -butyrate (lane 4), R13A/R14A (lane 5) and a C‑terminal HA‑tagged -construct (lane 6). (A, right panel) Immunoblot of HA‑hIK1 (20 mg total protein) and HA‑C‑Term (2 mg total protein). Reduced incubation temperature (27°C) increases cell surface expression of RKR/AAA‑HA‑hIK1. HA‑C‑Term was not detected during cell surface IP confirming that only cell surface epitope is recognized. Similar results were obtained in three separate experiments. (B) Average DCEBIO‑stimulated, clotrimazole‑sensitive current density (pA/pF) for each construct at ‑20 mV. The number of experiments is indicated in parenthesis. Asterisks indicate statistical significance (p < 0.05).