Figure 6.

Patches from HEK293 cells stably transfected with either (A) HA‑hIK1 or (B) RKR/AAA cells are excised into a bath containing 10 mM free Ca²⁺. The -channels are further activated with 10 mM DCEBIO. The bath is then switched to 200 nM Ca²⁺ and subsequently exposed to 10 mM DCEBIO. (B) The RKR/AAA channels cannot reactivate at low Ca²⁺. (C) DCEBIO‑stimulated increase in current for wild-type (n = 17) and RKR/AAA (n = 7) hIK1 in the -presence of either 10 mM (blue bars) or 200 nM (green bars) free Ca²⁺.