Skip to main content
. 2012 May 1;7(5):544–546. doi: 10.4161/psb.19675

graphic file with name psb-7-544-g1.jpg

Figure 1. Helja is not glycosylated. A purified fraction of Helja was obtained from sunflower extracellular fluids (EF) collected from 10 g of seeds as previously described20 and subsequent purification on a D-mannose-agarose chromatography.6 Twenty μg of the mannose-eluted fraction (Helja) was analyzed in a 12% SDS-PAGE and sequentially stained with GelCode Glycoprotein dye (Pierce) (A) and Coomassie brilliant blue (B). Radish peroxidase was used as positive glycosylation control (+) and soybean trypsin inhibitor as a negative control (-). The molecular weight markers are indicated on the left.