Analysis of the Temporal Expression Pattern of PhEXP1 by Semiquantitative Real-Time RT-PCR.
Total RNA was extracted from wild-type plants grown under standard greenhouse conditions. Analyses were performed with specific primers homologous to the 3′ untranslated region of PhEXP1.
(A) Temporal expression of PhEXP1 during petal development. RNA was extracted from petals sampled at stages 4 to 13 of P. hybrida flower development as described by Reale et al. (2002).
(B) P. hybrida var Mitchell flowers at stages 7 to 13 of development.
(C) Temporal expression of PhEXP1 during limb and tube development. RNA was extracted from petals sampled at stages 7 to 13 of P. hybrida flower development.
Error bars represent standard deviation.