Figure 9.

Line 25 Petal Limb Cell Walls Are Cellulose Deficient.

(A) FTIR analysis of petal limb cell walls. PCA was performed using 15 FTIR spectra from the wild type and line 25. PC1 explained 68.3% of the variance between spectra from wild-type plants and spectra from line 25 plants.

(B) The PC1 loading showed positive peaks characteristic of cellulose in the fingerprinting region (1158, 1099, 1060, and 1030 cm⁻¹), indicating that line 25 cell walls were deficient in cellulose relative to the wild type. Two other major peaks (1654 and 1550 cm⁻¹) appeared negatively in this PC loading. These peaks corresponded to amide groups of proteins, suggesting an enrichment of proteins in line 25 walls relative to the wild type.