Table 1.
Defense-Related Genes Differentially Expressed in WRKY70 Transgenic Plants
| Probe | Gene code | A/C | S/C | Function/Similarity | W box (TTGAC[C/T]) | W-like box (TTGACA) |
|---|---|---|---|---|---|---|
| Group I | ||||||
| 17068_at | At5g60900 | N | 2.5 | S-receptor kinase homolog 2 precursor | 3 | 1 |
| 16360_at | At4g21380 | N | 2.6 | Receptor-like Ser/Thr protein kinase ARK3 | 3 | 1 |
| 15616_s_at | At1g21250 | N | 2.7 | Wall-associated kinase 1 | 1 | 1 |
| 12364_at | At3g57240 | 1.8 | 2.8 | Glycosyl hydrolase family 17 (β-1,3-glucanase BG3) | 1 | 1 |
| 16620_s_at | At5g57560 | −2.2 | 2.9 | Xyloglucan endotransglycosylase TCH4 | 3 | 1 |
| 14638_at | At3g49120 | N | 3.1 | Peroxidase | 0 | 4 |
| 16054_s_at | At1g02920 | 1.8 | 3.5 | Glutathione S-transferase GST11 | 8 | 0 |
| 13432_at | At2g25000 | 1.8 | 3.6 | WRKY60 | 3 | 6 |
| 19181_s_at | At4g02380 | N | 3.7 | Senescence-associated SAG21 | 4 | 2 |
| 13659_at | At4g23150 | −1.9 | 3.8 | Ser/Thr kinase-like protein | 1 | 0 |
| 16365_at | At2g32680 | 1.5 | 4.4 | Similar to Leu-rich repeat disease resistance protein Cf-2.2 | 2 | 0 |
| 20446_s_at | At1g05570 | N | 4.5 | Callose synthase CALS1 (1,3-β-glucan synthase) | 3 | 0 |
| 12497_at | At2g31880 | N | 4.7 | Putative Leu-rich repeat transmembrane protein kinase | 6 | 0 |
| 16366_at | At4g04220 | N | 5.0 | Similar to Leu-rich repeat disease resistance protein Hcr2-2A | 6 | 2 |
| 14636_s_at | At1g75040 | N | 5.2 | Thaumatin-like protein | 3 | 1 |
| 18968_at | At5g57550 | 1.8 | 5.9 | Xyloglucan endotransglycosylase XTR3 | 4 | 1 |
| 20238_g_at | At3g13790 | N | 6.1 | Glycosyl hydrolase family 32, identical to β-fructofuranosidase 1 | 3 | 4 |
| 19195_at | At2g44380 | N | 6.5 | Putative CHP-rich zinc finger protein | 1 | 2 |
| 13187_i_at | At1g45145 | N | 8.1 | Thioredoxin | 7 | 1 |
| 12925_s_at | At4g29940 | 1.7 | 9.0 | Pathogenesis-related homeodomain protein PRHA | 0 | 2 |
| 20625_at | At3g48080 | N | 9.2 | Similar to disease resistance protein EDS1 | 0 | 3 |
| 13212_s_at | At3g57260 | N | 12.6 | Acidic β-1,3-glucanase 2 (BGL2), PR2 | 2 | 0 |
| 20345_at | At4g01700 | N | 13.1 | Glycosyl hydrolase family 19, similar to type II chitinase | 2 | 0 |
| 17840_at | At2g43570 | 1.8 | 18.4 | Glycosyl hydrolase family 19, similar to chitinase class IV | 3 | 1 |
| Group II | ||||||
| 19267_s_at | At4g02330 | −2.9 | 2.2 | Similar to pectinesterase | 5 | 1 |
| 16597_s_at | At3g24310 | −3.6 | N | Myb family transcription factor MYB71 | 0 | 0 |
| 19025_at | At1g77280 | −4.2 | −2.3 | Similar to receptor-like protein kinase | 2 | 1 |
| 16875_at | At4g23240 | −4.2 | 1.7 | Ser/Thr kinase-like protein | 4 | 3 |
| 16840_at | At1g30640 | −4.3 | N | Strong similarity to protein kinase | 2 | 2 |
| Group III | ||||||
| 20227_s_at | At1g52030 | 2.5 | 1.7 | Myrosinase binding protein MBP1.2 | 1 | 3 |
| 15125_f_at | At5g24780 | 4.7 | −1.8 | Vegetative storage protein VSP1 | 3 | 2 |
| 15141_s_at | At5g24770 | 5.2 | −1.9 | Vegetative storage protein VSP2 | 2 | 2 |
| Group IV | ||||||
| 18359_at | At3g57710 | N | −2.6 | Wall-associated kinase WAK4 | 2 | 0 |
| 15581_s_at | At2g28190 | N | −2.8 | Copper/zinc superoxide dismutase CSD2 | 2 | 1 |
| 18253_s_at | At1g76680 | N | −2.9 | 12-Oxophytodienoate reductase OPR1 | 2 | 0 |
| 18853_at | At4g21760 | N | −2.9 | Glycosyl hydrolase family 1; β-glucosidase | 5 | 1 |
| 12251_at | At2g34930 | N | −3.0 | Similar to Leu-rich repeat disease resistance protein Cf-2.1 | 4 | 0 |
| 18035_at | At1g53160 | N | −3.1 | Putative transcription factor | 0 | 0 |
| 18743_f_at | At5g07690 | N | −3.4 | Myb family transcription factor | 2 | 1 |
| 18156_at | At4g18640 | N | −3.4 | Putative Leu-rich repeat transmembrane protein kinase | 1 | 2 |
| 14606_at | At2g32990 | N | −3.6 | Glycosyl hydrolase family 9, similar to endo-β-1,4-glucanase | 2 | 1 |
| 17517_at | At5g36910 | N | −3.7 | Thionin THI2.2 | 2 | 1 |
The table indicates ≥2.5-fold upregulated or downregulated gene expression in antisense suppression line A18 (A/C) or in overexpression line S55 (S/C) compared to vector control pCP60. W box or W-like box distribution is within 1.5-kb promoter sequences. N, <1.5-fold difference.