Figure 5. FAVL elevation is associated with Chromosomal Instability and cisplatin sensitivity. (A) Both control and FAVL-overexperssion cells were treated with a series of MMC for 5 d. Cell viability was plotted upon the readings from MTT assay on cells treated for 5 d with difference concentrations of MMC, with non-treated cells as 100%. The images shown on the right are a set of cells fixed and stained with crystal blue. Cells carrying FACL are more sensitive to cell death triggered by MMC. (B) The same set of HTB-4 derivative cells were split and subsequently treated with 100 ng/ml MMC for 24 h. Both control and FAVL-overexpressing cells were prepared for chromosomal spread study. The percentage of chromosomal abnormalities was scored only upon breaks and tri/tetra radio-dials, which are FA-like chromosomal abnormalities. FAVL elevation enhances the percentage of FA-like chromosomal changes. Images shown are the whole or partial chromosome spreads prepared from treated cells.