Table 1.

Concentration of Ascorbate and Glutathione in the Incubation Medium of H. vulgare Aleurone Layers as Determined by Enzyme-Coupled Assays and HPLC

		Concentration (μM)		Method
	Time	ABA-Treated	GA-Treated	Enzyme Assay	ECD
Total ascorbate	21 h	0.0 ± 0.0	0.0 ± 0.0	*
	48 h	0.0 ± 0.0	0.0 ± 0.0	*
Reduced ascorbate	24 h	0.0 ± 0.0	0.0 ± 0.0		*
Total glutathione	24 h	0.8 ± 0.0	0.8 ± 0.1	*
	48 h	0.1 ± 0.1	0.5 ± 0.2	*
GSH	24 h	0.0 ± 0.0	0.5 ± 0.2		*
GSSH	24 h	0.5 ± 0.6	0.7 ± 0.5		*

Layers were incubated with either ABA or GA for the times indicated. The amounts of total ascorbate (reduced plus oxidized) and total glutathione were determined using enzyme-coupled assays. The amounts of reduced ascorbate, reduced glutathione (GSH), and fully oxidized glutathione (GSSH) were determined using an HPLC system equipped with an electrochemical detector (ECD). Each value is the mean ±sd of four flasks of aleurone layers.