Table 1.
RFs in Homozygous and Hemizygous Plants Containing Either a uidA (IC1, IC6, and IC9) or a Luciferase (58F) Gene as Recombination Substrate
Line | RFhemi | RFhomo | RFhomo:RFhemi |
---|---|---|---|
IC1 | 0.07 ± 0.01 | 0.32 ± 0.15 | 4.2 ± 1.2 |
IC6 | 0.08 ± 0.03 | 0.29 ± 0.09 | 3.9 ± 0.3 |
IC9 | 0.07 ± 0.04 | 0.18 ± 0.10 | 3.1 ± 0.9 |
58F | 3.1 ± 1.0 | 8.5 ± 2.3 | 2.8 ± 0.2 |
Hemizygous plants were generated by three independent outcrosses. Seeds homozygous and hemizygous for the recombination substrate were harvested from the same donor/recipient plants used for the corresponding crosses. The hemizygous and corresponding homozygous plants were assayed for somatic HR events at different time periods. Independent experiments were conducted three times (except for line 58F) using >80 plants per replicate. RFhemi, RF in hemizygous plants; RFhomo, RF in homozygous plants; RFhomo:RFhemi, ratio between RFs in homozygous and hemizygous plants.