Table 2.
RFs in Homozygous and Hemizygous Plants after Treatment with MMS, UV-B Radiation, UV-C Radiation, or BLM
| RFhemi
|
RFhomo
|
RFhomo:RFhemi
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Line | Type of Treatment | Untreated | Treated | Enh | Untreated | Treated | Enh | Untreated | Treated |
| IC1 | MMS | 0.05 ± 0.01 | 0.36 ± 0.05 | 7.2 ± 0.5 | 0.20 ± 0.02 | 1.18 ± 0.10 | 5.9 ± 0.5 | 4.1 ± 0.4 | 3.3 ± 0.2 |
| UV-B | 0.05 ± 0.01 | 0.08 ± 0.02 | 1.6 ± 0.1 | 0.16 ± 0.01 | 0.35 ± 0.04 | 2.1 ± 0.1 | 3.2 ± 0.3 | 4.5 ± 0.6 | |
| UV-C | 0.02 ± 0.01 | 0.04 ± 0.01 | 2.3 ± 0.5 | 0.04 ± 0.02 | 0.17 ± 0.03 | 5.1 ± 1.4 | 2.0 ± 0.1 | 4.2 ± 0.1 | |
| BLM | 0.01 | 0.05 | 5.0 | 0.01 | 0.14 | 14.0 | 1.1 | 2.7 | |
| IC6 | MMS | 0.07 ± 0.01 | 0.44 ± 0.06 | 6.6 ± 0.4 | 0.22 ± 0.03 | 1.40 ± 0.11 | 6.8 ± 0.1 | 3.1 ± 0.1 | 3.1 ± 0.1 |
| UV-B | 0.06 ± 0.01 | 0.11 ± 0.02 | 1.8 ± 0.1 | 0.20 ± 0.02 | 0.39 ± 0.04 | 1.9 ± 0.1 | 3.3 ± 0.2 | 3.4 ± 0.1 | |
| UV-C | 0.02 ± 0.01 | 0.08 ± 0.02 | 4.7 ± 1.3 | 0.05 ± 0.02 | 0.20 ± 0.03 | 4.5 ± 1.1 | 2.5 ± 0.0 | 2.5 ± 0.2 | |
| BLM | 0.01 | 0.03 | 3.0 | 0.009 | 0.08 | 8.9 | 0.9 | 2.4 | |
| IC9 | MMS | 0.03 ± 0.01 | 0.51 ± 0.05 | 18.5 ± 4.2 | 0.09 ± 0.01 | 1.67 ± 0.14 | 17.4 ± 0.4 | 3.2 ± 0.4 | 3.3 ± 0.0 |
| UV-B | 0.02 ± 0.01 | 0.20 ± 0.02 | 13.1 ± 6.3 | 0.09 ± 0.01 | 0.42 ± 0.04 | 4.7 ± 0.1 | 5.5 ± 1.3 | 2.1 ± 0.1 | |
| UV-C | 0.02 ± 0.01 | 0.11 ± 0.05 | 5.5 ± 0.2 | 0.05 ± 0.02 | 0.30 ± 0.04 | 6.8 ± 1.3 | 2.5 ± 0.2 | 2.8 ± 0.2 | |
| BLM | 0.008 | 0.03 | 3.7 | 0.02 | 0.08 | 4.0 | 2.5 | 2.7 | |
The determination of RFs in homozygous and hemizygous lines IC1, IC6, and IC9 was performed using 170 to 370 plants per experiment. Experiments were duplicated except for BLM treatment. Histochemical GUS assay was performed 4 d after each treatment. RFhemi, RF in hemizygous plants; RFhomo, RF in homozygous plants; Enh, average enhancement (±SD) of HRF after treatment; RFhomo:RFhemi, ratio between RFs in homozygous and hemizygous plants.