Table 2.
Oligonucleotide primers used for the qRT-PCR analysis
| Gene names | Accession No. | Primers | Product | |
|---|---|---|---|---|
|
GAPDH |
NM_001034034.1 |
Forward |
AAGGCCATCACCATCTTCCA |
280 - 299 |
| Reverse |
CCACCACATACTCAGCACCAGCAT |
355 - 332 |
||
|
bCSH1 |
NM_181007.2 |
Forward |
TGCCACACCGAATTCATGAC |
447 - 466 |
| Reverse |
AGGGCTTCGTCCTCTGTATTTG |
514 - 493 |
||
|
bPRP-1 |
NM_174159.2 |
Forward |
CACGGAGCTGCAGCATATGA |
501 - 520 |
| Reverse |
CCTTGTGGCGCTTGATAGGA |
558 - 539 |
||
|
bPAG1 |
NM_174411.2 |
Forward |
TCCACTTTCCGGCTTACCAA |
375 - 394 |
| Reverse |
CCTTTCATTCTCCCAGATCCAT |
436 - 415 |
||
|
IFNT |
NM_001015511.2 |
Forward |
CTAGGTGCCAGGCAGAACCT |
173 - 192 |
| Reverse |
GGGATGAGGAGAGAGTCTGTTCA |
232 - 210 |
||
|
bERVE-A |
NW_001493691.1 |
Forward |
GGATCTGACGGGAGACACAAA |
703321-703301 |
| Reverse |
CACCAATCCGGGAATCTTCA |
703281-703260 |
||
|
bERVE-B |
NW_001493674.2 |
Forward |
GGCCCAAGCACTCCTTCAT |
3100010-3099992 |
| Reverse |
CGCCCTTTTTCCCATTTCTT |
3099973-3099954 |
||
| BERV-K1 env |
AB587259.1 |
Forward |
GGAAATCACCGGGATGTCCT |
221-240 |
| Reverse |
GGAGAGGAGGCGCTTACCTG |
404-385 |
||
| BERV-K2 env |
AB587260.1 |
Forward |
AAAGGAGGTCAGGCCGCCTG |
221-240 |
| Reverse | TGGGGGAGGAGGCGCTTACCT | 406-386 | ||