Table 4.
Virus levels in blood and tissues at time of euthanasia
| Animal number |
Plasma |
PBMC |
Spleen |
Ax LN |
Ing LN |
Mes LN |
Thymus |
Tonsil |
Jejunum |
Colon |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RNA | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | DNA | RNA | |
| 29276 |
<15 |
4 |
<2 |
2 |
1 |
3 |
2 |
3 |
<2 |
<1 |
<1 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
| 30577 |
< 10 |
7 |
<2 |
<3 |
<3 |
4 |
<1 |
4 |
<1 |
7 |
<2 |
<2 |
<2 |
3 |
<1 |
<2 |
<2 |
2 |
<2 |
| 32186 |
20 |
15 |
<1 |
5 |
<1 |
40 |
9 |
7 |
90 |
30 |
200 |
<70 |
160 |
300 |
13,500 |
60 |
7 |
10 |
50 |
| 33088 |
< 10 |
6 |
<1 |
2 |
<1 |
8 |
10 |
4 |
1 |
5 |
3 |
<2 |
<2 |
3 |
1 |
<2 |
<2 |
<4 |
<4 |
| 33091 | 530,000 | 280 | 470 | 370 | 5,700 | 320 | 13,000 | 340 | 11,000 | 520 | 10,000 | <2 | 50 | 570 | 30,000 | 60 | 640 | 50 | 160 |
Cell-free levels in plasma are expressed as RNA copies per ml plasma. Cell-associated levels are expressed as number of viral RNA and DNA copies per 100,000 cell equivalents (determined by total copy number of CCR5 divided by two). < indicates below limit of detection (based on the detection limit of 30 viral RNA or DNA copies per reaction and the cell equivalents for the particular sample).
Ax LN = axillary lymph node, Ing LN = inguineal lymph node; Mes LN = mesenteric lymph node; NA = not available.