Figure 3.

RNA Expression of AGD2 and ALD1.

Tissue from the wild type and/or aldT-1 was used for RNA extraction and RNA gel blot analysis.

(A) ALD1 and PR1 expression in the fourth and fifth leaves of 20-d-old wild-type (Col) and agd2-1 plants.

(B) ALD1 and PR1 expression in wild-type (Ws) and ald1-T1 plants. Leaves (fourth and fifth) from 18-d-old plants were infected with P. s. maculicola DG3 (OD₆₀₀ = 0.01).

(C) Expression of AGD2 and ALD1 during development of the fourth leaf. Leaf length and age of the plant when RNA was extracted are indicated.

(D) Expression of AGD2 and ALD1 in the indicated tissues.

(E) A time course of steady state AGD2 mRNA accumulation. Twenty-day-old plants were kept in a 16-h-light/8-h-dark (16L/8D) cycle or were shifted to continuous dark (D) for the indicated time after 4 h in the light of the normal light cycle (16-h-light/8-h-dark) and then switched back to light (L) for 1 or 4 h. Although the signal was low for the ALD1 transcript, the probe was verified to be high specific activity. It gave a strong signal on the blots probed in parallel (see [A] and [B]).