Table 3.
Substrate | Cosubstrate (mM) | Km (mM) | kcat (s−1) | kcat/Km (mM−1 s−1) | |
---|---|---|---|---|---|
AGD2 | Lys | 2-oxoglutarate (100 mM) | 58.8 | 5.33 | 0.091 |
AGD2P398S | Lys | 2-oxoglutarate (100 mM) | 71.4 | 2.87 | 0.040 |
ALD1 | Lys | 2-oxoglutarate (50 mM) | 0.53 | 4.42 | 8.34 |
AGD2 | 2-oxoglutarate | Lys (100 mM) | 1.82 | 2.83 | 1.55 |
AGD2P398S | 2-oxoglutarate | Lys (100 mM) | 1.92 | 1.83 | 0.95 |
ALD1 | 2-oxoglutarate | Lys (100 mM) | 2.63 | 3.75 | 1.43 |
AGD2 | Ala | 2-oxoglutarate (100 mM) | 25.6 | 1.67 | 0.065 |
AGD2P398S | Ala | 2-oxoglutarate (100 mM) | 23.3 | 0.87 | 0.037 |
ALD1 | Ala | 2-oxoglutarate (100 mM) | 5.56 | 2.15 | 0.39 |
AGD2 | Arg | 2-oxoglutarate (100 mM) | 154 | 0.69 | 0.0045 |
AGD2P398S | Arg | 2-oxoglutarate (100 mM) | 167 | 0.35 | 0.0021 |
ALD1 | Arg | 2-oxoglutarate (100 mM) | 5.10 | 2.07 | 0.41 |
Km values were obtained from the initial velocity data and Lineweaver-Burk plots. kcat was calculated using equation Vmax = kcat [Enzyme]. Three independent measurements were averaged.