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Kinetic Parameters of Recombinant AGD2- and ALD1-Catalyzed Reactions for Ala Synthesis Using Glutamate and Pyruvate as Cosubstrates
| Substrate | Cosubstrate (mM) | Km (mM) | kcat (s−1) | kcat/Km (mM−1 s−1) | |
|---|---|---|---|---|---|
| AGD2 | Glutamate | Pyruvate (50 mM) | 3.08 | 6.47 | 2.10 |
| AGD2P398S | Glutamate | Pyruvate (50 mM) | 3.12 | 1.48 | 0.47 |
| ALD1 | Glutamate | Pyruvate (50 mM) | 37.0 | 5.80 | 0.16 |
Km values were obtained from the initial velocity data and Lineweaver-Burk plots. Three independent measurements were averaged.
