Figure 3.

Endogenous GA Levels at 4 and 22°C in Dark-Imbibed Seeds.

As illustrated in Figure 2A, wild-type seeds were imbibed in the dark, irradiated with an FR light pulse, and then incubated in the dark at 22 or 4°C for 96 h until harvested. Data are means with standard errors from three measurements using independent seed batches unless otherwise indicated. The asterisks indicate means from two measurements (with similar results). Note that the y axis scale for 13-nonhydroxylated GAs (top; GA_{12,15,24,9,4,34,51}) and that for 13-hydroxylated GAs (bottom; GA_{53,44,19,20,1,8,29}) are different for clarity. gdw, grams dry weight; n.d., detectable but could not be quantified because of comigration of impurities.