Figure 5.

Effects of Cold Treatment on Germination of Wild-Type and ga4-2 Mutant Seeds.

(A) Diagram showing temperature and light conditions. Wild-type and ga4-2 mutant seeds were imbibed in the dark, irradiated with an FR light pulse (hatched box), and then incubated in the dark at 4°C for 48 h before being placed at 22°C for 72 h in the dark to allow germination (+ cold). As a control, the incubation at 4°C for 48 h was omitted (− cold). Seeds also were imbibed at 22°C under continuous white light. The triangle indicates the start time of imbibition.

(B) Germination rates under conditions described in (A). Asterisk, no germination (0%).