Table 1.
Expression of GA Biosynthesis Genes in Dry Seeds versus Seeds after Cold Treatment
| Signal Valuea
|
||||||
|---|---|---|---|---|---|---|
| Enzyme | Gene | Probe Set | AGI Gene Codeb | Dry Seeds | Seeds After Cold Treatment | Differential Expressionc |
| ent-copalyl diphosphate synthase | AtCPS1 | 17498_s_at | At4g02780 | NDd | ND | |
| ent-kaurene synthase | AtKS1 | 15301_s_at | At1g79460 | ND | ND | |
| ent-kaurene oxidase | AtKO1 | 17543_s_at | At5g25900 | 35.1 | 36.4 | |
| ent-kaurenoic acid oxidase | AtKAO1 | 20493_at | At1g05160 | 15.8 | 2.4 | |
| AtKAO2 | 13021_at | At2g32440 | 8.2 | 7.1 | ||
| GA 20-oxidase | AtGA20ox1 | 17299_s_at | At4g25420 | ND | 3.4 | Yes |
| AtGA20ox2 | 17739_at | At5g51810 | ND | 1.7 | ||
| AtGA20ox3 | 12368_at | At5g07200 | ND | 13.7 | Yes | |
| GA 3-oxidase | AtGA3ox1 | 17549_at | At1g15550 | ND | 63.4 | Yes |
| AtGA3ox2 | 17492_at | At1g80340 | ND | 2.3 | ||
| AtGA3ox3 | 19250_at | At4g21690 | ND | ND | ||
| GA 2-oxidase | AtGA2ox1 | 16292_at | At1g78440 | ND | ND | |
| AtGA2ox2 | 13789_at | At1g30040 | ND | 2.1 | Yes | |
| AtGA2ox3 | 19480_s_at | At2g34500 | ND | ND | ||
a
Signal values from a single replicate are shown.
b
Arabidopsis Genome Initiative (2000).
c
Genes are classified as being differentially expressed when signal values deviate fourfold or more in duplicated experiments.
d
The signal is not detectable (ND) by microarray analysis.