Figure 1.

FLJ10540 was highly correlated with osteopontin expression in human NPC specimens. (A) The microarray expression patterns of FLJ10540 in NPC patients were normalized against the expression patterns on HG_U133 plus 2.0 chips. The boxplot shows the data distribution as a grouping classification and indicates that there was a statistically significant difference (p<0.0001) between the tumor tissues and the normal tissues from NPC patients. (B) The mRNA expression levels of FLJ10540 and osteopontin from twelve NPC patients and five normal tissues were determined by Q-RT-PCR. The results were normalized against the expression levels of GAPDH mRNA in each sample. N: normal tissues; T: tumor tissues. (C) Western blotting analysis of FLJ10540 and osteopontin expressions were performed in seven NPC samples and two normal tissues. Total proteins were extracted from respective tissues and probed with antibodies against FLJ10540 and osteopontin. β-actin was used as a control.