Figure 5.

Osteopontin reversed the cell growth inhibition of siFLJ10540-NPC. (A and B, left panel) A negative control (NC) siRNA plus one different FLJ10540 siRNA (siFLJ10540) were transfected into TW01 and Hone1 cells for 24 hours. After transfection, Western blotting was performed using anti-FLJ10540 and β-actin antibodies. (A and B, right panels) Using the same panel, the siFLJ10540 transfectants and NC with or without osteopontin (OPN) stimulation were seeded into 96-well plates with 1.0% of FBS. The cells were cultured for 1–3 days followed by MTT assay (OD570) to quantitate cell growth. The data were normalized against the OD570 value on day 1 of each treatment. The growth curve of TW01 and Hone1 cells are shown as the mean ±s.d. of three independent experiments.