Figure 6.

FLJ10540 alone or increased FLJ10540, mediated by osteopontin stimulation modulated cell migration and invasion in NPC cells. (A and B) For the migration assays, 5 × 10³ cells (Vehicle-TW01, FLJ10540-1-TW01, FLJ10540-2-TW01, negative control, and siFLJ10540-TW01) with or without osteopontin (OPN) stimulation were seeded into the top of a Transwell insert. After 24 hours, the cells on top were scraped, and the cells that had migrated to the bottom were fixed and stained with Giemsa. For the invasion assays, 1 × 10⁴ cells were seeded after the addition of Matrigel. The relative-fold migration/invasion values for the stable clones were normalized against the vehicle/negative control cells and are represented diagrammatically. All of the data represent the mean ± s.d. of three independent experiments.